Cholpisit Ice Kiattisewee

Cholpisit Ice Kiattisewee

he/him

Engineering living therapeutics to combat antimicrobial resistance

Publications

You can also find my articles on my Google Scholar profile.

CRISPRi-assisted E. coli strains increase success rate of burdensome construct cloning

Published in bioRxiv (preprint), 2026

A bioRxiv preprint describing CRISPRi cloning strains that suppress burdensome genetic constructs during assembly.

Recommended citation: Faulkner I., Kiattisewee C., Darst B., Leejareon P., Yoshikuni Y., Zalatan J.G.†, Carothers J.M.† (2026). "CRISPRi-assisted E. coli strains increase success rate of burdensome construct cloning." bioRxiv. DOI: 10.64898/2026.06.02.729553. Preprint. https://doi.org/10.64898/2026.06.02.729553

Conjugation-based genome engineering enables rapid prototyping and bioproduction in non-model bacteria

Published in bioRxiv (preprint), 2026

A bioRxiv preprint describing a broad-host-range platform for iterative genome engineering in non-model bacteria.

Recommended citation: Guzman M.S., Kiattisewee C., Comes J., Robert A.M., Cardiff R.A.L., Cook M., et al. (2026). "Conjugation-based genome engineering enables rapid prototyping and bioproduction in non-model bacteria." bioRxiv. DOI: 10.64898/2026.04.17.717921. Preprint. https://doi.org/10.64898/2026.04.17.717921

Engineering Biology: International Guidelines for Constructing Higher Education Curricula

Published in UNESCO Publishing, 2026

International guidance for developing higher-education curricula in engineering biology.

Recommended citation: Chaiyen P., Fahmi A., Khomfoi S., Kiattisewee C. (2026). "Engineering Biology: International Guidelines for Constructing Higher Education Curricula." UNESCO Publishing. https://www.unesco.org/en/articles/engineering-biology-international-guidelines-constructing-higher-education-curricula

Systematic Mapping of Bacterial CRISPRa Systems for Synergistic Gene Activation Reveals Antagonistic Effects

Published in ACS Synthetic Biology, 2025

A systematic comparison of bacterial transcriptional activators and their combinations across engineered promoters.

Recommended citation: Kiattisewee C.+, Karanjia A.V.+, Cardiff R.A.L.+, Olander K.E., Leejareon P., Alvi S.S., Carothers J.M.†, Zalatan J.G.† (2025). "Systematic Mapping of Bacterial CRISPRa Systems for Synergistic Gene Activation Reveals Antagonistic Effects." ACS Synthetic Biology. 14(8):3232-3244. DOI: 10.1021/acssynbio.5c00358. https://doi.org/10.1021/acssynbio.5c00358

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling

Published in Nature Communications, 2024

This paper is part of Ice PhD research at UW.

Recommended citation: Fontana J.+, Sparkman-Yager D.+, Faulkner I.+, Cardiff R., Kiattisewee C., Walls A., Primo T.G., Kinnunen P.C., Garcia Martin H., Zalatan J.G.†, Carothers J.M.† (2024). "Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling." Nature Communications. 15:6341. PMID: 39068154 https://doi.org/10.1038/s41467-024-50528-1

CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications

Published in Annual Review of Chemical and Biomolecular Engineering, 2024

Ice and team at UW crafted this extensive review of current bacterial CRISPR technologies.

Recommended citation: Alba Burbano D.+, Kiattisewee C.+, Karanjia A.V., Cardiff R.A.L., Faulkner I.D., Sugianto W., Carothers J.M.† (2024). "CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications." Annual Review of Chemical and Biomolecular Engineering. 15(1):389-430. https://doi.org/10.1146/annurev-chembioeng-100522-114706

Enzymes, auxiliaries and cells for the recycling and upcycling of polyethylene terephthalate (PET)

Published in Current Opinion in Systems Biology, 2024

Ice helps the VISTEC team with this review mainly on the microbial perspective.

Recommended citation: Wongsatit T.+, Srimora T.+, Kiattisewee C.†, Uttamapinant C.† (2024). "Enzymes, auxiliaries and cells for the recycling and upcycling of polyethylene terephthalate (PET)." Current Opinion in Systems Biology. 38:100515. https://doi.org/10.1016/j.coisb.2024.100515

Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits

Published in Proceedings of the National Academy of Sciences of the United States of America, 2023

This paper is part of Ice PhD research at UW.

Recommended citation: Alba Burbano D.+, Cardiff R.A.L.+, Tickman B.I., Kiattisewee C., Maranas C.J., Zalatan J.G.†, Carothers J.M.† (2023). "Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits." Proceedings of the National Academy of Sciences of the United States of America. 120(30):e2220358120. PMID: 37463216. https://doi.org/10.1073/pnas.2220358120

Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction

Published in Materials Today Bio, 2023

This paper is part of Ice PhD research at UW.

Recommended citation: Sugianto W., Altin-Yavuzarslan G., Tickman B.I., Kiattisewee C., Yuan S.F., Brooks S.M., Wong J., Alper H.S., Nelson A., Carothers J.M.† (2023). "Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction." Materials Today Bio. 20:100677. PMID: 37273790. https://www.sciencedirect.com/science/article/pii/S2590006423001370

Effect of UV light irradiation on the In2O3 nanoparticle films synthesis by the sparking method

Published in Materials Letters, 2023

Ice was working with a team at Chiang Mai University, a friend from undergraduate time

Recommended citation: Siriariyachai R., Sirithuwanon N., Kantarak E., Sroila W., Kumpika T., Kiattisewee C., Limpichaipanit A., Singjai P., Wiranwetchayan O.† (2023). "Effect of UV light irradiation on the In2O3 nanoparticle films synthesis by the sparking method." Materials Letters. 134046. https://doi.org/10.1016/j.matlet.2023.134046

Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants

Published in ACS Synthetic Biology, 2022

This paper is part of Ice PhD research at UW.

Recommended citation: Kiattisewee C.+, Karanjia A.V.+, Legut M., Daniloski Z., Koplik S.E., Nelson J., Kleinstiver B.P., Sanjana N.E., Carothers J.M.†, Zalatan J.G.† (2022). "Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants." ACS Synthetic Biology. 11(12):4103-4112. PMID: 36378874. https://doi.org/10.1021/acssynbio.2c00405

Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems

Published in Cell Systems, 2022

This paper is part of Ice PhD research at UW.

Recommended citation: Tickman B.I.+, Burbano D.A.+, Chavali V.P., Kiattisewee C., Fontana J., Khakimzhan A., Noireaux V., Zalatan J.G.†, Carothers J.M.† (2022). "Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems." Cell Systems. 13(3):215-229.e8. PMID: 34800362. https://www.cell.com/cell-systems/fulltext/S2405-4712(21)00419-1

Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida

Published in Metabolic Engineering, 2021

This paper is part of Ice PhD research at UW.

Recommended citation: Kiattisewee C., Dong C., Fontana J., Sugianto W., Peralta-Yahya P., Carothers J.M.†, Zalatan J.G.† (2021). "Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida." Metabolic Engineering. 66:283-295. PMID: 33930546. https://www.sciencedirect.com/science/article/pii/S109671762100063X

Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments

Published in Angewandte Chemie International Edition, 2021

This paper is part of Ice work as a research assistant at VISTEC.

Recommended citation: Pongpamorn P.+, Kiattisewee C.+, Kittipanukul N.+, Jaroensuk J., Trisrivirat D., Maenpuen S., Chaiyen P.† (2021). "Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments." Angewandte Chemie International Edition. 60(11):5749-5753. PMID: 33247515. https://doi.org/10.1002/anie.202013962

Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements

Published in Nature Communications, 2020

This paper is part of Ice PhD research at UW.

Recommended citation: Fontana J.+, Dong C.+, Kiattisewee C., Chavali V.P., Tickman B.I., Carothers J.M.†, Zalatan J.G.† (2020). "Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements." Nature Communications. 11(1):1618. PMID: 32238808. https://doi.org/10.1038/s41467-020-15454-y

One-Pot Bioconversion of l-Arabinose to l-Ribulose in an Enzymatic Cascade

Published in Angewandte Chemie International Edition, 2019

This paper is part of Ice work as a research assistant at VISTEC.

Recommended citation: Chuaboon L., Wongnate T., Punthong P., Kiattisewee C., Lawan N., Hsu C.Y., Lin C.H., Bornscheuer U.T., Chaiyen P.† (2019). "One-Pot Bioconversion of l-Arabinose to l-Ribulose in an Enzymatic Cascade." Angewandte Chemie International Edition. 58(8):2428-2432. PMID: 30605256. https://doi.org/10.1002/anie.201814219

Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway

Published in Journal of Biological Chemistry, 2019

This paper is part of Ice work as a research assistant at VISTEC.

Recommended citation: Jaroensuk J., Intasian P., Kiattisewee C., Munkajohnpon P., Chunthaboon P., Buttranon S., Trisrivirat D., Wongnate T., Maenpuen S., Tinikul R., Chaiyen P.† (2019). "Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway." Journal of Biological Chemistry. 294(30):11536-11548. PMID: 31182484. https://www.jbc.org/article/S0021-9258(20)30656-6/fulltext

Kinetic studies of conjugate addition of amines to allenic and acrylic esters and their correlation with antibacterial activities against Staphylococcus aureus

Published in Monatshefte für Chemie - Chemical Monthly, 2018

This paper is part of Ice undergraduate and master research at Mahidol University.

Recommended citation: Kiattisewee C., Kaidad A., Jiarpinitnun C., Luanphaisarnnont T.† (2018). "Kinetic studies of conjugate addition of amines to allenic and acrylic esters and their correlation with antibacterial activities against Staphylococcus aureus." Monatshefte für Chemie - Chemical Monthly. 149:1059–1068. https://doi.org/10.1007/s00706-018-2175-1