CRISPRi-assisted E. coli strains increase success rate of burdensome construct cloning
Published in bioRxiv (preprint), 2026
Abstract
This preprint presents CRISPR interference-assisted cloning strains that reduce expression from common promoters and ribosome-binding sites while burdensome constructs are assembled in E. coli. Genome-integrated CRISPR components and customizable guide RNAs provide a practical route for improving difficult cloning workflows before constructs are transferred to their intended hosts.
Recommended citation: Faulkner I., Kiattisewee C., Darst B., Leejareon P., Yoshikuni Y., Zalatan J.G.†, Carothers J.M.† (2026). "CRISPRi-assisted E. coli strains increase success rate of burdensome construct cloning." bioRxiv. DOI: 10.64898/2026.06.02.729553. Preprint. https://doi.org/10.64898/2026.06.02.729553