Conjugation-based genome engineering enables rapid prototyping and bioproduction in non-model bacteria
Published in bioRxiv (preprint), 2026
Abstract
This preprint introduces conjugation-based serine recombinase-assisted genome engineering (cSAGE), a broad-host-range platform designed for predictable, iterative genomic integration in transformation-resistant bacteria. The approach combines conjugative DNA delivery, low-copy vectors, orthogonal recombinases, and modular genetic parts to support pathway assembly and cross-host benchmarking.
Recommended citation: Guzman M.S., Kiattisewee C., Comes J., Robert A.M., Cardiff R.A.L., Cook M., et al. (2026). "Conjugation-based genome engineering enables rapid prototyping and bioproduction in non-model bacteria." bioRxiv. DOI: 10.64898/2026.04.17.717921. Preprint. https://doi.org/10.64898/2026.04.17.717921