Systematic Mapping of Bacterial CRISPRa Systems for Synergistic Gene Activation Reveals Antagonistic Effects

Abstract

This study systematically characterizes targeting rules for several bacterial CRISPR activation systems in a shared promoter context. Testing combinations at their optimal target positions did not produce synergistic activation and frequently revealed antagonistic effects, highlighting important mechanistic differences between bacterial and eukaryotic transcriptional activation.

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Recommended citation: Kiattisewee C.⁺, Karanjia A.V.⁺, Cardiff R.A.L.⁺, Olander K.E., Leejareon P., Alvi S.S., Carothers J.M.^†, Zalatan J.G.^† (2025). "Systematic Mapping of Bacterial CRISPRa Systems for Synergistic Gene Activation Reveals Antagonistic Effects." ACS Synthetic Biology. 14(8):3232-3244. DOI: 10.1021/acssynbio.5c00358. https://doi.org/10.1021/acssynbio.5c00358