Cholpisit Ice Kiattisewee

Cholpisit Ice Kiattisewee

he/him

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A list of all the posts and pages found on the site. For you robots out there is an XML version available for digesting as well.

Pages

Posts

Future Blog Post

Published:

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Blog Post number 4

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 3

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 2

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 1

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

publications

A 1,2-Addition Pathway for C(sp2)—H Activation at a Dinickel Imide

Published in Chemistry – A European Journal, 2017

This paper is part of an undergraduate internship at Purdue University.

Recommended citation: Powers I.G., Kiattisewee C., Mullane K.C., Schelter E.J., Uyeda C.† (2017). "A 1,2-Addition Pathway for C(sp2)—H Activation at a Dinickel Imide." Chemistry – A European Journal. 23(32):7694-7697. PMID: 28453895

Kinetic studies of conjugate addition of amines to allenic and acrylic esters and their correlation with antibacterial activities against Staphylococcus aureus

Published in Monatshefte für Chemie - Chemical Monthly, 2018

This paper is part of Ice undergraduate and master research at Mahidol University.

Recommended citation: Kiattisewee C., Kaidad A., Jiarpinitnun C., Luanphaisarnnont T.† (2018). "Kinetic studies of conjugate addition of amines to allenic and acrylic esters and their correlation with antibacterial activities against Staphylococcus aureus." Monatshefte für Chemie - Chemical Monthly. 149:1059–1068.

Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway

Published in Journal of Biological Chemistry, 2019

This paper is part of Ice work as a research assistant at VISTEC.

Recommended citation: Jaroensuk J., Intasian P., Kiattisewee C., Munkajohnpon P., Chunthaboon P., Buttranon S., Trisrivirat D., Wongnate T., Maenpuen S., Tinikul R., Chaiyen P.† (2019). "Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway." Journal of Biological Chemistry. 294(30):11536-11548. PMID: 31182484.

One-Pot Bioconversion of l-Arabinose to l-Ribulose in an Enzymatic Cascade

Published in Angewandte Chemie International Edition, 2019

This paper is part of Ice work as a research assistant at VISTEC.

Recommended citation: Chuaboon L., Wongnate T., Punthong P., Kiattisewee C., Lawan N., Hsu C.Y., Lin C.H., Bornscheuer U.T., Chaiyen P.† (2019). "One-Pot Bioconversion of l-Arabinose to l-Ribulose in an Enzymatic Cascade." Angewandte Chemie International Edition. 58(8):2428-2432. PMID: 30605256.

Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments

Published in Angewandte Chemie International Edition, 2021

This paper is part of Ice work as a research assistant at VISTEC.

Recommended citation: Pongpamorn P.+, Kiattisewee C.+, Kittipanukul N.+, Jaroensuk J., Trisrivirat D., Maenpuen S., Chaiyen P.† (2021). "Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments." Angewandte Chemie International Edition. 60(11):5749-5753. PMID: 33247515..

Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems

Published in Cell Systems, 2022

This paper is part of Ice PhD research at UW.

Recommended citation: Tickman B.I.+, Burbano D.A.+, Chavali V.P., Kiattisewee C., Fontana J., Khakimzhan A., Noireaux V., Zalatan J.G.†, Carothers J.M.† (2022). "Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems." Cell Systems. 13(3):215-229.e8. PMID: 34800362.

Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants

Published in ACS Synthetic Biology, 2022

This paper is part of Ice PhD research at UW.

Recommended citation: Kiattisewee C.+, Karanjia A.V.+, Legut M., Daniloski Z., Koplik S.E., Nelson J., Kleinstiver B.P., Sanjana N.E., Carothers J.M.†, Zalatan J.G.† (2022). "Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants." ACS Synthetic Biology. 11(12):4103-4112. PMID: 36378874.

Effect of UV light irradiation on the In2O3 nanoparticle films synthesis by the sparking method

Published in Materials Letter, 2023

Ice was working with a team at Chiang Mai University, a friend from undergraduate time

Recommended citation: Siriariyachai R., Sirithuwanon N., Kantarak E., Sroila W., Kumpika T., Kiattisewee C., Limpichaipanit A., Singjai P., Wiranwetchayan O.† (2023). "Effect of UV light irradiation on the In2O3 nanoparticle films synthesis by the sparking method." Materials Letters. 134046.

Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction

Published in Materials Today Bio, 2023

This paper is part of Ice PhD research at UW.

Recommended citation: Sugianto W., Altin-Yavuzarslan G., Tickman B.I., Kiattisewee C., Yuan S.F., Brooks S.M., Wong J., Alper H.S., Nelson A., Carothers J.M.† (2023). "Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction." Materials Today Bio. 20:100677. PMID: 37273790.

Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits

Published in Proceedings of the National Academy of Sciences of the United States of America, 2023

This paper is part of Ice PhD research at UW.

Recommended citation: Alba Burbano D.+, Cardiff R.A.L.+, Tickman B.I., Kiattisewee C., Maranas C.J., Zalatan J.G.†, Carothers J.M.† (2023). "Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits." Proceedings of the National Academy of Sciences of the United States of America. 120(30):e2220358120. PMID: 37463216.

CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications

Published in Annual Review in Chemical and Biomolecular Engineering, 2024

Ice and team at UW crafted this extensive review of current bacterial CRISPR technologies.

Recommended citation: Alba Burbano D.+, Kiattisewee C.+, Karanjia A.V., Cardiff R.A.L., Faulkner I.D., Sugianto W., Carothers J.M.† (2024). "CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications." Annual Review in Chemical and Biomolecular Engineering. 15, 11.1-11.42

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling

Published in Nature Communications, 2024

This paper is part of Ice PhD research at UW.

Recommended citation: Fontana J.+, Sparkman-Yager D.+, Faulkner I.+, Cardiff R., Kiattisewee C., Walls A., Primo T.G., Kinnunen P.C., Garcia Martin H., Zalatan J.G.†, Carothers J.M.† (2024). "Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling." Nature Communications. 15:6341. PMID: 39068154

talks

National Delegate at the Asian Science Camp 2013

Published:

Ice participated in the Asian Science Camp in Japan as a national delegate from Thailand. Here is how excited a freshman undergrad look like seeing a Nobel Laureat (Ada Yonate) for the first time!!!

Investigating promoter distance rules for effective bacterial CRISPRa with distinct transcriptional activation mechanisms

Published:

EBRC Annual Meeting 2022 was held at UC Berkeley, California, for this year after coming back from COVID. Ice was supported by EBRC as a board member of Student and Postdoc Association (SPA) and presented a poster at this conference. It was such a great opportunity to attend an in-person conference with scientists in the field. Apart from my research poster, I am also in charge of our DEI initiatives poster!

Design rules for multiple bacterial CRISPRa systems enable synergistic gene activation

Published:

ASBMB Annual Meeting or DiscoverBMB is a big scientific conference in Biochemistry and Molecular Biology. Ice presented orally in the Session: New mechanisms for transcriptional control of gene expression. As a local Seattleite, Ice acted as a host to show friends around the town!!! Especially for good food~~~
An abstract of this work presented at ASBMB annual meeting 2023 is available in a special issue of J. Biol. Chem.

Invited to ACS BRIDGE Career Kickstarter Workshop

Published:

ACS BRIDGE hosted a Career Kick-Starter workshop for professional development. Ice was invited to the workshop to foster DEI initiave across chemical sciences. With great experience from the CKS workshop, Ice is bringing the workshop to UW with sponsorship from UW Department of Chemistry!!!

teaching

Get Rich with SynBio

Podcast, Biology Beyond Nature team, Thailand, 2021

Ice co-founded a biweekly Podcast series under the theme “Get Rich with SynBio” or “รวยด้วย SynBio”. This initiative is a collaboration of diverse Thai SynBio researchers and science communicators around the world. We discussed about potential SynBio and Biotech movements accounted up to 26 episodes across 2 years.

STEM Grad School 101 Panel

Panel, MolES DEI Committee, 2021

Ice co-founded the MolES DEI Committee with friends in the program to reach out to broader community that might be interested in Molecular Engineering and Sciences. One of those events is the panel on STEM Grad School starting from how grad school is like to prepare for grad school. The recordings of our panels were available on MolES’s youtube channel.

Bacterial Synthetic Biology

Lectures, Vidyasirimedhi Institute of Science and Technology, 2022

Ice gave a lecture on bacterial synthetic biology for graduate students at VISTEC.

Synthetic Biology & Genome-Scale Engineering 2022

Lectures, Naresuan University, 2022

Ice gave two invited lectures through the SynBio course provided at Naresuan University in Thailand.
His first lecture is on CRISPR Technologies

and his second lecture is on Living Therapeutics

Research Mentorship

Mentor, University of Washington, 2023

Ice mentored students to perform Basic Molecular Biology and Microbiology to design and develop CRISPR tool for bacterial engineering Throughout his Ph.D., Ice have mentored 2 undergraduate students, 1 Master’s student, 1 international visiting Master’s student and 12 early career Ph.D. students

EBRC Panel 2023

Panel, EBRC SPA Team, 2023

Together with EBRC-SPA team, especially Ross Jones, Ice co-lead the panel series focusing on “Introduction to Graduate School in Engineering Biology” for Engineering Biology students. The recordings of our panels were available on EBRC’s youtube channel.

SynBio4ALL Intro Course 2023

Virtual course, SynBio4ALL Team, 2023

Ice co-founded the educational initiative for African students focusing on Synthetic Biology. He was in charge of the asynchronous learning using his experience from being a youtuber!!! More information can be found at the SynBio4ALL website Ice taught Lecture 5 focusing on Laboratory Techniques in Synthetic Biology

and also co-taught Lecture 6 with Heidi in the 2nd part focusing on CRISPR Technologies

EBRC In Translation

Podcast, EBRC, USA, 2024

Ice worked with the Podcast team at “EBRC In Translation” founded among EBRC Student and Postdocs.