CV

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Education

Ph.D in Molecular Engineering and Sciences (Data Science Option), 2018-2023
- University of Washington, Seattle, WA, USA
- GPA: 3.79/4.00
- Distinguished Dissertation Award
M.S. in Chemistry (International Program), 2016-2017
- Mahidol University, Bangkok, Thailand
- GPA: 4.00/4.00
- Dean’s List and Outstanding Thesis Award
B.S. in Chemistry, 2012-2016
- Mahidol University, Bangkok, Thailand
- GPA: 3.89/4.00
- First Class Honors

Research/Work experience

2023 – Present: Postdoctoral Scholar
- Continuing Ph.D. Research
2018 – 2023: Ph.D. Student at Center for Synthetic Biology, Institute of Molecular Engineering and Sciences, University of Washington, WA, USA
- Advisors: Jesse Zalatan: Dept. of Chemistry and James Carothers: Dept. of Chemical Engineering) collaborating with interdisciplinary team at UW and national laboratories (PNNL, LBNL, and ORNL)
  - Bacterial CRISPR transcriptional modulations tool development
  - Metabolic Engineering for carbon-conservation in multiple bacteria (e.g. Pseudomonas putida, Bacillus subtilis, Acinetobacter baylyi, etc.)
  - Model-driven DBTL cycle acceleration by tunable CRISPR programs
  - Genome-wide investigation of gene regulatory circuits with CRISPRa/i
2017 – 2018: Research Assistant at School of Biomolecular Science and Engineering, Vidhyasirimethi Institute of Technology (VISTEC), Thailand
- Advisor: Pimchai Chaiyen
  - Metabolic pathway engineering in E. coli for biofuel synthesis
  - New chemistry of Carboxylic Acid Reductase (CAR) enzyme
2016 – 2017: Master Student at Department of Chemistry, Faculty of Science, Mahidol University, Thailand
- Advisor: Torsak Luanphaisarnnont and Co-advisor: Chutima Jiarpinitnun
  - Kinetic investigation of conjugate addition reactions into allenic esters
  - Antibacterial activity tests of allenic esters against Staphylococcus aureus

Skills

Molecular Biology
Microbiology
- Unconventional bacteria
  - Pseudomonas putida
  - Acinetobacter baylyi
  - Bacillus subtilis
    - Vibrio natriegens
    - Shewanella oneidensis
    - Rhodobacter sphaeroides
    - Rhodopseudomonas palustris
    - Cupriviadus necator
Protein Engineering
- Protein Purification
- Enzyme Characterization
Synthetic Chemistry

Patents

U.S. Patent Application No. 18/306960. Filed: April 24, 2023. Based on Provisional Patent Application No. 63/335,143 Filed: April 26, 2022. GENETIC MANIPULATION METHOD IN BACTERIA. Inventors: Cholpisit KIATTISEWEE, James M. CAROTHERS, Jesse ZALATAN, Ian D. FAULKNER, Jason FONTANA, Chen DONG
PCT-WO2019209187 A1: “A luciferase reporter system and an assay for gene expression profiling using the same” April 25th, 2018. Inventors: Pimchai CHAIYEN, Juthamas JAROENSUK, Cholpisit KIATTISEWEE, Jittima PHONBUPPHA, Thanyaporn WONGNATE, Ruchanok TINIKUL, Nattanon AKERATCHATAPAN
DIP-1801005261: “ระบบรายงานผลเอนไซม์เปล่งแสงและการวัดการแสดงออกของยีนจากวิธีการนั้น” April 25th, 2018. Inventor: พิมพ์ใจ ใจเย็น, จุฑามาส เจริญสุข, ชลพิสิฐ เกียรติเสวี, จิตติมา พลบุบผา, ธัญญพร วงศ์เนตร, รัชนก ตินิกุล, ณัฐ นนท์ เอกรัชตพันธุ์
DIP-1801002500: “วิธีการสังเคราะห์ไรบูโลสโดยเอนไซม์ไพราโนสทูออกซิเดส” April 27th, 2018. Inventor: พิมพ์ใจ ใจเย็น, ลิตวดี เจือบุญ, ธัญญพร วงศ์เนตร, ชลพิสิฐ เกียรติเสวี, แป้งร่ำ ปั้นทอง
DIP-1801003340: “ระบบวิศวกรรมเมตาบอลิกสำหรับการผลิตสารไฮโดรคาร์บอนในเซลล์เจ้าบ้าน” June 7th, 2018. Inventor: พิมพ์ใจ ใจเย็น, จุฑามาส เจริญสุข, ชลพิสิฐ เกียรติเสวี, ภัทรวรรณ อินทเศียร, ดวงทิพย์ ไตรศรีวิรัตน์, พบธรรม มั่นขจรพงษ์, สุภชา บุตรานนท์
DIP-1801005184: “กระบวนการสังเคราะห์เอสเทอร์” filed on August 30th, 2018. Inventor: พิมพ์ใจ ใจเย็น, ชลพิสิฐ เกียรติเสวี, ภรณ์กนกส์ พงษ์ภมร, จุฑามาส เจริญสุข, ณรงค์ยศ กิตติภาณุกุล, ดวงทิพย์ ไตรศรีวิรัตน์

Publications

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling

Fontana J.⁺, Sparkman-Yager D.⁺, Faulkner I.⁺, Cardiff R., Kiattisewee C., Walls A., Primo T.G., Kinnunen P.C., Garcia Martin H., Zalatan J.G.^†, Carothers J.M.^† (2024). "Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling." Nature Communications. 15:6341. PMID: 39068154

CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications

Alba Burbano D.⁺, Kiattisewee C.⁺, Karanjia A.V., Cardiff R.A.L., Faulkner I.D., Sugianto W., Carothers J.M.^† (2024). "CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications." Annual Review in Chemical and Biomolecular Engineering. 15, 11.1-11.42

Enzymes, auxiliaries and cells for the recycling and upcycling of polyethylene terephthalate (PET)

Wongsatit T.⁺, Srimora T.⁺, Kiattisewee C.^†, Uttamapinant C.^† (2024). "Enzymes, auxiliaries and cells for the recycling and upcycling of polyethylene terephthalate (PET)." Current Opinion in System Biology. 38, 100515

Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits

Alba Burbano D.⁺, Cardiff R.A.L.⁺, Tickman B.I., Kiattisewee C., Maranas C.J., Zalatan J.G.^†, Carothers J.M.^† (2023). "Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits." Proceedings of the National Academy of Sciences of the United States of America. 120(30):e2220358120. PMID: 37463216.

Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction

Sugianto W., Altin-Yavuzarslan G., Tickman B.I., Kiattisewee C., Yuan S.F., Brooks S.M., Wong J., Alper H.S., Nelson A., Carothers J.M.^† (2023). "Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction." Materials Today Bio. 20:100677. PMID: 37273790.

Effect of UV light irradiation on the In₂O₃ nanoparticle films synthesis by the sparking method

Siriariyachai R., Sirithuwanon N., Kantarak E., Sroila W., Kumpika T., Kiattisewee C., Limpichaipanit A., Singjai P., Wiranwetchayan O.^† (2023). "Effect of UV light irradiation on the In₂O₃ nanoparticle films synthesis by the sparking method." Materials Letters. 134046.

Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants

Kiattisewee C.⁺, Karanjia A.V.⁺, Legut M., Daniloski Z., Koplik S.E., Nelson J., Kleinstiver B.P., Sanjana N.E., Carothers J.M.^†, Zalatan J.G.^† (2022). "Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants." ACS Synthetic Biology. 11(12):4103-4112. PMID: 36378874.

Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems

Tickman B.I.⁺, Burbano D.A.⁺, Chavali V.P., Kiattisewee C., Fontana J., Khakimzhan A., Noireaux V., Zalatan J.G.^†, Carothers J.M.^† (2022). "Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems." Cell Systems. 13(3):215-229.e8. PMID: 34800362.

Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida

Kiattisewee C., Dong C., Fontana J., Sugianto W., Peralta-Yahya P., Carothers J.M.^†, Zalatan J.G.^† (2021). "Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida." Metabolic Engineering. 66:283-295. PMID: 33930546.

Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments

Pongpamorn P.⁺, Kiattisewee C.⁺, Kittipanukul N.⁺, Jaroensuk J., Trisrivirat D., Maenpuen S., Chaiyen P.^† (2021). "Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments." Angewandte Chemie International Edition. 60(11):5749-5753. PMID: 33247515..

Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements

Fontana J.⁺, Dong C.⁺, Kiattisewee C., Chavali V.P., Tickman B.I., Carothers J.M.^†, Zalatan J.G.^† (2020). "Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements." Nature Communications. 11(1):1618. PMID: 32238808.

One-Pot Bioconversion of l-Arabinose to l-Ribulose in an Enzymatic Cascade

Chuaboon L., Wongnate T., Punthong P., Kiattisewee C., Lawan N., Hsu C.Y., Lin C.H., Bornscheuer U.T., Chaiyen P.^† (2019). "One-Pot Bioconversion of l-Arabinose to l-Ribulose in an Enzymatic Cascade." Angewandte Chemie International Edition. 58(8):2428-2432. PMID: 30605256.

Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway

Jaroensuk J., Intasian P., Kiattisewee C., Munkajohnpon P., Chunthaboon P., Buttranon S., Trisrivirat D., Wongnate T., Maenpuen S., Tinikul R., Chaiyen P.^† (2019). "Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway." Journal of Biological Chemistry. 294(30):11536-11548. PMID: 31182484.

Kinetic studies of conjugate addition of amines to allenic and acrylic esters and their correlation with antibacterial activities against Staphylococcus aureus

Kiattisewee C., Kaidad A., Jiarpinitnun C., Luanphaisarnnont T.^† (2018). "Kinetic studies of conjugate addition of amines to allenic and acrylic esters and their correlation with antibacterial activities against Staphylococcus aureus." Monatshefte für Chemie - Chemical Monthly. 149:1059–1068.

A 1,2-Addition Pathway for C(sp²)—H Activation at a Dinickel Imide

Powers I.G., Kiattisewee C., Mullane K.C., Schelter E.J., Uyeda C.^† (2017). "A 1,2-Addition Pathway for C(sp²)—H Activation at a Dinickel Imide." Chemistry – A European Journal. 23(32):7694-7697. PMID: 28453895

Investigating promoter distance rules for effective bacterial CRISPRa with distinct transcriptional activation mechanisms

May 19, 2022

Poster at EBRC Annual Meeting 2022, Berkeley, California

Kinetic studies of conjugate addition of amines to allenic and acrylic esters and their correlation with antibacterial activities against Staphylococcus aureus

November 05, 2017

Poster at ACS Asia-Pacific International Chapters Conference 2017, Jeju, South Korea

National Delegate at the 67^th Lindau Nobel Laureate Meeting

June 25, 2017

Nobel Laureate Meeting at The 67^th Lindau Nobel Laureate Meeting, Lindau, Germany

National Delegate at the Asian Science Camp 2013

August 25, 2013

Nobel Laureate Meeting at Asian Science Camp 2013, Tsukuba, Japan

Peer Review Services (and Assisting in Peer-Review)

Nucleic Acids Research – August 2023 (Direct invitation to C.K.)
Nucleic Acids Research – 4 articles – April 2023 (Assisting J.M.C.), March 2022 (Assisting J.M.C.), December 2020 (Assisting J.G.Z.), and September 2019 (Assisting J.G.Z.)
Nature Communications – 3 articles – December 2022 (Assisting J.G.Z.), October 2022 (Assisting J.G.Z.), and March 2020 (Assisting J.M.C.)
ACS Synthetic Biology – 2 articles – October 2023 (Assisting J.G.Z.) and February 2023 (Assisting J.G.Z.)
Nature Microbiology – February 2024 (Assisting J.M.C.)
Nature Chemical Biology – November 2023 (Assisting J.M.C.)
Cell Reports Methods – May 2023 (Assisting J.M.C.)
Metabolic Engineering Communications – May 2022 (Assisting J.M.C.)
The CRISPR Journal – June 2020 (Assisting J.G.Z.)
Microbial Cell Factory – February 2020 (Assisting J.M.C.)
Biotechnology Journal – March 2018 (Assisting P.C.)

Leadership and Services

2023 – Present
- “Membership and Engagement Co-Chair”, Engineering Biology Research Consortium Student and Postdoctoral Association (EBRC-SPA)
2024 – Present
- Mentor at ASM Future Leaders Mentorship Fellowship (FLMF) Program
2022 – 2023
- UW MolES Graduate Student Association (MolES-GSA), Member & Cohort Representative
2021 – 2023
- “Membership and Outreach Co-Chair” and “DEI Task Force Liaison”, Engineering Biology Research Consortium Student and Postdoctoral Association (EBRC-SPA)
2023
- Panelist at DiscoverBMB Community Day at the ASBMB annual conference
2021 – 2022
- UW Graduate School Interdisciplinary Committee (GSIC), as a MolES Student Liaison working on developing new course curriculum for Ph.D. students
2021 – 2022
- รวยด้วย SynBio a.k.a. Get Rich with SynBio, a Scientific Communication Series in Thai language on Clubhouse, Thailand Synthetic Biology Consortium
2020 – 2022
- Co-founder of the MolES Diversity, Equity, and Inclusion (DEI) Committee – Organizational Subcommittee member
2020 – Present
- Member of the EBRC (Engineering Biology Research Consortium) Student and Postdoc Association (SPA): Focus on Roadmapping:
  - Engineering Biology for Climate & Sustainability 2023
  - Engineering Biology 2019 Assessment

Honors and Awards

2023
- Distinguished Dissertation Award 2023, Molecular Engineering and Sciences Institute (MolES), University of Washington
2023
- Invited to attend ACS BRIDGE Career Kickstarter Conference 2023
2023
- ACS Graduate Student and Postdoctoral Scholar Recognition Program 2023, Leadership in Mentoring, one of 21 Graduate Student Recipients
2023
- AIChE Foundation registration support and DEI travel award for SEED 2023
2022
- Travel Award, Graduate Student Symposium “Mother (Nature) Knows Best: Bonding with Nature’s Proteins”, ACS Meeting Spring 2022, San Diego, USA
2019
- Outstanding Thesis Awards 2019, Faculty of Graduate Studies, Mahidol University
2017
- National delegate to participate the Lindau Nobel Laureate Meetings 67th (2017) dedicated to Chemistry, Lindau, Germany

Cholpisit Ice Kiattisewee

CV